In a three-necked flask, 91.6 mL of phosphate buffer (0.2 mol/L, pH = 7. 0) and 7.5 mL of phenol solution (0.8 mol/L) were added in sequence. The microwave ultrasonic combiner was turned on, the microwave power was adjusted to 550 W, and the temperature of the reaction system was heated to 75 °C. Subsequently, the power was reduced to 100 W, and the temperature of the reaction system was maintained at 75 ° C by controlling the flow rate of the cooling water while adjusting the ultrasonic wave so that the ultrasonic power density of the reaction system was 5 W /cm 2 . Then, a certain amount of the above-mentioned immobilized enzyme was added to the above reaction system, and thoroughly mixed under stirring at 200 r /min, and rapidly added 0. 4 mL of hydrogen peroxide solution (0.3 mol/L), and the reaction bottle was taken after being fully reacted. The supernatant was filtered through a micropore filter to measure the residual phenol concentration.