A high performance liquid chromatography-tandem mass spectrometric method for the determination of nonylphenol and octylphenol in children's toys was established. The sample was extracted with methanol by microwave, using ZORBAX Eclipse Plus C18 column, methanol-water as mobile phase, separated by gradient elution, and detected by tandem quadrupole mass spectrometry multiple reaction monitoring method to retain time and relative ion abundance (ion Ratio) Qualitative, external standard method quantitative. Under the optimized conditions, the method can complete the analysis of the analyte within 5 min.
The linear relationship between nonylphenol and octylphenol in the range of 0.001~0.10 mg/L is good, the correlation coefficient is greater than 0.999, and the lower limit of quantification of the method is 0.02 mg/kg. When the spiked levels were 0.02, 0.2, 2.0 mg/kg, the average spiked recoveries of the analytes ranged from 76.8 % to 99.0 %, and the relative standard deviations (n = 6) were less than 6.6%. The method is accurate, rapid and sensitive, and is suitable for rapid confirmation and quantitative detection of nonylphenol and octylphenol in children's toys.